RFLP
Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) is a method used to distinguish organisms based on patterns from their DNA cleavage. It works by analyzing the length of DNA fragments after digestion with restriction enzymes. If the distance between cleavage sites differs in two organisms, the resulting fragment lengths will vary. These differences in fragment patterns can be used to differentiate species or even strains. Polymorphisms, which are inherited differences in at least 1% of the population, are key to this analysis. Applications of RFLP RFLP has several practical uses. In forensic science, it is applied in paternity tests and criminal investigations to identify DNA sources. It is also used in medical genetics, specifically to detect known mutations associated with diseases. Additionally, RFLP helps in measuring recombination rates, contributing to the creation of genetic maps by calculating the distance between RFLP loci i...